HCS based Cell Sorting System

Cell Sorting

Sorting and obtaining target biological species such as live cells from complex mixtures are critical for biotechnology research, diagnostics, and therapeutics. In laboratory, cell sorting is performed by fluorescence-activated cell sorting(FACS). However, FACS is inherently limited in sorting cells based on the observation of cellular features. Applications such as cell-based therapeutics, stem cell culturing, and in-cell directed evolution of functional macro-molecules all require the enrichment of target cells from complex mixtures. For these cases, image-based discrimination and sorting must be performed simultaneously at the single cell level. We propose to meet this need by utilizing recent advances in imaging technology and computer-based image processing.